基因数据处理54之bwa-mem运行paird-end（1千万条100bp的reads）
2017-12-18 01:14
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指令：
记录：
``` hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub/cs-bwamem$ bwa mem GRCH38BWAindex/GRCH38chr1L3556522.fasta g38L100c10000000Nhs20Paired1.fq g38L100c10000000Nhs20Paired2.fq >g38L100c10000000Nhs20Paired12.bwamem.sam ```
记录：
``` [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.56, 9.99) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.288 CPU sec, 28.176 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44726, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.53, 9.96) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.613 CPU sec, 28.511 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44694, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.342 CPU sec, 28.226 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44749, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.58, 9.89) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.540 CPU sec, 28.448 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44527, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.274 CPU sec, 27.164 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44737, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.49, 9.93) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.131 CPU sec, 27.037 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44754, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.50, 9.91) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.950 CPU sec, 28.852 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44754, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.55, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.460 CPU sec, 28.383 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44775, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.45, 9.96) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.181 CPU sec, 28.064 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44772, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.649 CPU sec, 26.550 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44687, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.48, 9.96) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.146 CPU sec, 27.040 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44724, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.46, 9.97) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.986 CPU sec, 27.901 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44784, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.42, 9.96) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.150 CPU sec, 28.055 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44763, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.49, 9.93) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.879 CPU sec, 27.774 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44761, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.46, 9.97) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.319 CPU sec, 28.204 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44644, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.48, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.493 CPU sec, 28.389 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44764, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.832 CPU sec, 27.726 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44731, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.655 CPU sec, 27.560 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44747, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.50, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.473 CPU sec, 26.357 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44705, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.46, 9.93) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.065 CPU sec, 26.959 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44775, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.52, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.701 CPU sec, 26.588 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44711, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.55, 9.93) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.542 CPU sec, 27.439 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44796, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.44, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.927 CPU sec, 26.810 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44733, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.47, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.880 CPU sec, 26.770 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44586, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.44, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.442 CPU sec, 27.327 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44797, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.55, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.669 CPU sec, 26.560 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44830, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.52, 10.02) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.317 CPU sec, 27.199 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44673, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.50, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.593 CPU sec, 26.494 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44680, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.47, 10.00) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.958 CPU sec, 28.860 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44757, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.43, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.593 CPU sec, 26.510 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44743, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.45, 10.01) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.088 CPU sec, 26.977 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44742, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.48, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.671 CPU sec, 26.563 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44717, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.46, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.961 CPU sec, 26.844 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44752, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.56, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.785 CPU sec, 26.680 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44636, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.54, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 29.157 CPU sec, 29.058 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44723, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.56, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.284 CPU sec, 28.187 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44749, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.52, 9.99) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.546 CPU sec, 27.446 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44801, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.58, 9.91) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.707 CPU sec, 28.604 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44725, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.57, 9.96) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.331 CPU sec, 28.219 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44734, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.52, 10.03) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.157 CPU sec, 28.055 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44748, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.45, 9.86) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.092 CPU sec, 27.990 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44720, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.49, 9.97) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.999 CPU sec, 26.903 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44713, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.48, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.041 CPU sec, 26.925 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44776, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.46, 9.87) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.063 CPU sec, 25.954 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44749, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.44, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.955 CPU sec, 26.843 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44779, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.47, 9.90) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.835 CPU sec, 26.727 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44698, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.53, 9.96) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.406 CPU sec, 27.299 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44703, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.53, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.740 CPU sec, 26.638 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44650, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.46, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.605 CPU sec, 27.498 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44700, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.52, 9.91) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.448 CPU sec, 26.338 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44678, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.47, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.126 CPU sec, 27.013 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44770, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.45, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.949 CPU sec, 26.841 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44680, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.48, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.292 CPU sec, 28.194 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44711, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.50, 9.90) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.177 CPU sec, 27.076 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44816, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.45, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.250 CPU sec, 27.134 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44805, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.46, 9.91) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.526 CPU sec, 26.418 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44715, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.49, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.488 CPU sec, 26.372 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44748, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.44, 9.88) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.081 CPU sec, 26.971 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44805, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.43, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.954 CPU sec, 26.837 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44676, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.52, 9.96) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.999 CPU sec, 26.891 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44754, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.46, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.633 CPU sec, 26.606 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44769, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.58, 9.91) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.897 CPU sec, 26.800 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44783, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.48, 9.93) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.218 CPU sec, 27.107 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44750, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.99) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.628 CPU sec, 26.529 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44792, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.528 CPU sec, 26.422 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44720, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.43, 9.93) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.523 CPU sec, 26.425 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44719, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.84) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.634 CPU sec, 26.515 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44608, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.50, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.356 CPU sec, 27.255 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44601, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.52, 9.91) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.313 CPU sec, 28.220 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44725, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.43, 10.00) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.932 CPU sec, 28.833 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44742, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.54, 9.89) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.259 CPU sec, 27.148 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44597, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.630 CPU sec, 28.536 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44703, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.55, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.028 CPU sec, 27.915 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44777, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.54, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.764 CPU sec, 27.682 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44795, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.45, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.550 CPU sec, 28.450 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44728, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.45, 9.96) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.730 CPU sec, 26.640 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44710, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.48, 10.00) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.746 CPU sec, 26.632 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44648, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 10.01) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.218 CPU sec, 27.114 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44799, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.45, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.441 CPU sec, 27.334 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44566, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.53, 10.00) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.266 CPU sec, 27.160 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44831, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.47, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 26.645 CPU sec, 26.533 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44674, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.45, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.178 CPU sec, 27.074 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44737, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.58, 9.96) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.949 CPU sec, 27.848 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44820, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.56, 9.91) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.635 CPU sec, 28.527 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44690, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.49, 9.95) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.851 CPU sec, 28.750 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44760, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.51, 9.97) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.416 CPU sec, 28.330 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44657, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.54, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.556 CPU sec, 28.445 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44619, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.48, 9.91) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.425 CPU sec, 28.330 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44811, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.42, 9.87) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.271 CPU sec, 28.155 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44672, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.49, 9.89) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.555 CPU sec, 28.451 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44757, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.47, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.800 CPU sec, 28.698 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44682, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.46, 9.92) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.281 CPU sec, 28.178 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44687, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.56, 9.98) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.502 CPU sec, 28.390 real sec [M::process] read 100000 sequences (10000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44812, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.47, 9.94) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 28.047 CPU sec, 27.942 real sec [M::process] read 12662 sequences (1266200 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44719, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 199, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (166, 231) [M::mem_pestat] mean and std.dev: (198.54, 10.01) [M::mem_pestat] low and high boundaries for proper pairs: (153, 244) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 100000 reads in 27.914 CPU sec, 27.865 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 5659, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 198, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (163, 233) [M::mem_pestat] mean and std.dev: (198.29, 10.17) [M::mem_pestat] low and high boundaries for proper pairs: (149, 247) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 12662 reads in 3.538 CPU sec, 3.471 real sec [main] Version: 0.7.13-r1126 [main] CMD: bwa mem GRCH38BWAindex/GRCH38chr1L3556522.fasta g38L100c10000000Nhs20Paired1.fq g38L100c10000000Nhs20Paired2.fq [main] Real time: 5117.394 sec; CPU: 5130.004 sec hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub/cs-bwamem$ ```
参考
【1】https://github.com/xubo245/AdamLearning 【2】https://github.com/bigdatagenomics/adam/ 【3】https://github.com/xubo245/SparkLearning 【4】http://spark.apache.org
研究成果：
【1】 [BIBM] Bo Xu, Changlong Li, Hang Zhuang, Jiali Wang, Qingfeng Wang, Chao Wang, and Xuehai Zhou, "Distributed Gene Clinical Decision Support System Based on Cloud Computing", in IEEE International Conference on Bioinformatics and Biomedicine. (BIBM 2017, CCF B) 【2】 [IEEE CLOUD] Bo Xu, Changlong Li, Hang Zhuang, Jiali Wang, Qingfeng Wang, Xuehai Zhou. Efficient Distributed Smith-Waterman Algorithm Based on Apache Spark (CLOUD 2017, CCF-C). 【3】 [CCGrid] Bo Xu, Changlong Li, Hang Zhuang, Jiali Wang, Qingfeng Wang, Jinhong Zhou, Xuehai Zhou. DSA: Scalable Distributed Sequence Alignment System Using SIMD Instructions. (CCGrid 2017, CCF-C). 【4】more: https://github.com/xubo245/Publications
Help
If you have any questions or suggestions, please write it in the issue of this project or send an e-mail to me: xubo245@mail.ustc.edu.cn Wechat: xu601450868 QQ: 601450868
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