基因数据处理30之avocado运行avocado-cli中的avocado问题1和2

println("stats.coverage:" + stats.coverage)

lazy val coverage = ComputingCoverage.time {
ScoreCoverage(inputDataset)
}

Exception in thread "main" java.lang.UnsupportedOperationException: empty collection
at org.apache.spark.rdd.RDD$$anonfun$reduce$1$$anonfun$apply$36.apply(RDD.scala:985)
at org.apache.spark.rdd.RDD$$anonfun$reduce$1$$anonfun$apply$36.apply(RDD.scala:985)
at scala.Option.getOrElse(Option.scala:120)
at org.apache.spark.rdd.RDD$$anonfun$reduce$1.apply(RDD.scala:985)
at org.apache.spark.rdd.RDDOperationScope$.withScope(RDDOperationScope.scala:147)
at org.apache.spark.rdd.RDDOperationScope$.withScope(RDDOperationScope.scala:108)
at org.apache.spark.rdd.RDD.withScope(RDD.scala:286)
at org.apache.spark.rdd.RDD.reduce(RDD.scala:965)
at org.bdgenomics.avocado.stats.ScoreCoverage$.apply(ScoreCoverage.scala:44)
at org.bdgenomics.avocado.stats.AvocadoConfigAndStats$$anonfun$coverage$1.apply(AvocadoConfigAndStats.scala:33)
at org.bdgenomics.avocado.stats.AvocadoConfigAndStats$$anonfun$coverage$1.apply(AvocadoConfigAndStats.scala:33)
at org.apache.spark.rdd.Timer.time(Timer.scala:57)
at org.bdgenomics.avocado.stats.AvocadoConfigAndStats.coverage$lzycompute(AvocadoConfigAndStats.scala:32)
at org.bdgenomics.avocado.stats.AvocadoConfigAndStats.coverage(AvocadoConfigAndStats.scala:32)
at org.bdgenomics.avocado.cli.Avocado.run(Avocado.scala:263)
at org.bdgenomics.avocado.cli.AvocadoSuite$.main(AvocadoSuite.scala:60)
at org.bdgenomics.avocado.cli.AvocadoSuite.main(AvocadoSuite.scala)
at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
at sun.reflect.NativeMethodAccessorImpl.invoke(Unknown Source)
at sun.reflect.DelegatingMethodAccessorImpl.invoke(Unknown Source)
at java.lang.reflect.Method.invoke(Unknown Source)
at com.intellij.rt.execution.application.AppMain.main(AppMain.java:144)

println("stats.referenceSeq:" + stats.referenceSeq)

println("stats.referenceSeq:" + stats.referenceSeq)

stats.referenceObservations:MapPartitionsRDD[26] at flatMap at SliceReference.scala:28
2016-05-28 15:16:50 ERROR Executor:96 - Exception in task 0.0 in stage 10.0 (TID 9)
java.io.NotSerializableException: org.bdgenomics.formats.avro.NucleotideContigFragment
Serialization stack:
- object not serializable (class: org.bdgenomics.formats.avro.NucleotideContigFragment, value: {"contig": {"contigName": "chrUn_KN707606v1_decoy", "contigLength": 2200, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": null}, "description": "AC:KN707606.1  gi:734691250  LN:2200  rl:unplaced  M5:20c768ac79ca38077e5012ee0e5f8333  AS:hs38d1", "fragmentSequence": "ctagtagctgggactacaagcgcccgccaccacacccggctaatttttttgtatttttagtggagacaggtttcaccgtgttggccaggatggtctcgatctcctgaccttgtgatctgcccaccttgccctcccaaagtgctgggattacaggcatgagccaccatacccggcagTGTCCTATCCATTTTTAAGGCAGCCACTTGGAGTTGGAGCATGTCTTTCTCTCATAATCTCTTACCAGATGTCTCAGAGCAGCCTGTGCACTTTAACTCCAGACATTCTGCCACTGAGCCCCCTAGAGCTCCAGCTTTTAAAGCACTTGGGGTGAGCCTCGAGAGATGACAGACGGAGCTGCCCAAGAGCTGCCAGCTGCCAACCCTGCCTGGGGCTTCACGGCCCGCGCCCTACTTCCTCTCAGCTGGCTCCACACCCTGGGGCGTGTAATTTCCAAATTCTCACTCCCAGGGCTAATTTGGGGGATAAGACATTTGATTAGAAGTATCAgaaaccagctgggcatggtggctcacacctgtaatcccagcactttgggaggttatgactagaggatcatttgaactcaggaattcaagaccagcctggataacagtgagaccccatctctacaaaatataaacaattatgtgagcatggtggtgcacacctgtagtccctgttccttgggaggctgaggccggaggatcccttgagcccaggagttcaaggctgcagagagctgcgattgtgccactgcacactaacctgggagatagagcaagaacttgtctcagaaaaaaaaagtatcaggaaCTAATCTCCAGTCCTATCAAGTTAGGCATAAGGTCAATGTGTGATAGCTGAGTGTCACAGAAACCAAGGACAGGAATGCAACTGCCACTGGGGATGAACTGGAAGTGGGGAGTTAAACCACCTCAGAATGTccccatttttgtttcttctccagATGTGCTGCTTTGCTTTTCCGTATGTTTCTCTACGGACCAGCTACCTCTCCTCTGCCAACAGATCCAAGTTGTGCATGTTATGGGTCCAAACACCACGTGACAAGCCCATTCTTCCAGTTTCTCAGACCAGAAACTGCACTGTCCTCTAACTGCTTCTTCTCCCTCTTGCATCTGGTCCTTGGGGAAATCCTGTTTGCCCGGCCTTCAGCATATATCCACAGTTTAACCTTAACCACTCCTCGCCACCACTCGCGGGGGCGAGCAGCCTTCGCCCCCTGCCTAGATTACTACAGTAACTTCATTGTTCTTTCTACTTCTCTCTTTGCCCCTCTGCTATCTCAAAACAGCATCCAAAATGCACCTAGCAAGAGCATGTCATTCCTCTGCACAAAACTCTccaacttctctctttttttttttttttttttttttgagacggagtctcactctgtcacccaggctggagtgcaatagtgtgatcttggctcactgcaacctccacctcccagattcaagcgattctcctgcctcagcctcctgagtagctgagattacaggttcatgtcaccatgcccggctaatttttgtatttttagtagagacagggtttcaccatgttagtcaggctggtctcgaactcctgaccttgtgatccacccgcctcagcctcccaaagtgctgggattataggcatgagccaccgtgcatgacCAACTTCTCTTTTTGTTCAGAGTAAAAGCCAACGGCCCATGAGGCTTTCCATGGTCACGCCTCCGCTCATTCGCTCTGTGGCTTTGTCTTACACGGGTTCACTCCTCACTGGCCGCCTTGCTGACCCCATAGCTCACGGGCCTTACTCTGCTctcggggcctttgcacttgctccaCTGCAAATGCTCCTCCCCCAGAGGCCTTTGTGGCCCATTCCCTCGGTTCCTTAGGAACAATCCCTTCCCTGGTCAAACCTCCACTGACATCTGTCTCCTtcccttctgaattttttttctccgGTAGTATTTATCACTCTGCTATCCTTAGGATTTCCTTATCTTGTTTATCATCATCTCCTCATCCAGAGcttaagtcctttttttttttttgagatagagtctcgctctgtcgcccaggctggagtgcagtggcgcgatctcgtctcgctgaaagctccacctcccgggttcacgccattctcccgcctcagcctcccgagtagctgggactacaggcactcg", "fragmentNumber": 0, "fragmentStartPosition": 0, "fragmentLength": 2200, "numberOfFragmentsInContig": 1})
- element of array (index: 0)
- array (class [Lorg.bdgenomics.formats.avro.NucleotideContigFragment;, size 1)
at org.apache.spark.serializer.SerializationDebugger$.improveException(SerializationDebugger.scala:40)
at org.apache.spark.serializer.JavaSerializationStream.writeObject(JavaSerializer.scala:47)
at org.apache.spark.serializer.JavaSerializerInstance.serialize(JavaSerializer.scala:81)
at org.apache.spark.executor.Executor$TaskRunner.run(Executor.scala:236)
at java.util.concurrent.ThreadPoolExecutor.runWorker(Unknown Source)
at java.util.concurrent.ThreadPoolExecutor$Worker.run(Unknown Source)
at java.lang.Thread.run(Unknown Source)
2016-05-28 15:16:50 ERROR TaskSetManager:75 - Task 0.0 in stage 10.0 (TID 9) had a not serializable result: org.bdgenomics.formats.avro.NucleotideContigFragment
Serialization stack:
- object not serializable (class: org.bdgenomics.formats.avro.NucleotideContigFragment, value: {"contig": {"contigName": "chrUn_KN707606v1_decoy", "contigLength": 2200, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": null}, "description": "AC:KN707606.1  gi:734691250  LN:2200  rl:unplaced  M5:20c768ac79ca38077e5012ee0e5f8333  AS:hs38d1", "fragmentSequence": "ctagtagctgggactacaagcgcccgccaccacacccggctaatttttttgtatttttagtggagacaggtttcaccgtgttggccaggatggtctcgatctcctgaccttgtgatctgcccaccttgccctcccaaagtgctgggattacaggcatgagccaccatacccggcagTGTCCTATCCATTTTTAAGGCAGCCACTTGGAGTTGGAGCATGTCTTTCTCTCATAATCTCTTACCAGATGTCTCAGAGCAGCCTGTGCACTTTAACTCCAGACATTCTGCCACTGAGCCCCCTAGAGCTCCAGCTTTTAAAGCACTTGGGGTGAGCCTCGAGAGATGACAGACGGAGCTGCCCAAGAGCTGCCAGCTGCCAACCCTGCCTGGGGCTTCACGGCCCGCGCCCTACTTCCTCTCAGCTGGCTCCACACCCTGGGGCGTGTAATTTCCAAATTCTCACTCCCAGGGCTAATTTGGGGGATAAGACATTTGATTAGAAGTATCAgaaaccagctgggcatggtggctcacacctgtaatcccagcactttgggaggttatgactagaggatcatttgaactcaggaattcaagaccagcctggataacagtgagaccccatctctacaaaatataaacaattatgtgagcatggtggtgcacacctgtagtccctgttccttgggaggctgaggccggaggatcccttgagcccaggagttcaaggctgcagagagctgcgattgtgccactgcacactaacctgggagatagagcaagaacttgtctcagaaaaaaaaagtatcaggaaCTAATCTCCAGTCCTATCAAGTTAGGCATAAGGTCAATGTGTGATAGCTGAGTGTCACAGAAACCAAGGACAGGAATGCAACTGCCACTGGGGATGAACTGGAAGTGGGGAGTTAAACCACCTCAGAATGTccccatttttgtttcttctccagATGTGCTGCTTTGCTTTTCCGTATGTTTCTCTACGGACCAGCTACCTCTCCTCTGCCAACAGATCCAAGTTGTGCATGTTATGGGTCCAAACACCACGTGACAAGCCCATTCTTCCAGTTTCTCAGACCAGAAACTGCACTGTCCTCTAACTGCTTCTTCTCCCTCTTGCATCTGGTCCTTGGGGAAATCCTGTTTGCCCGGCCTTCAGCATATATCCACAGTTTAACCTTAACCACTCCTCGCCACCACTCGCGGGGGCGAGCAGCCTTCGCCCCCTGCCTAGATTACTACAGTAACTTCATTGTTCTTTCTACTTCTCTCTTTGCCCCTCTGCTATCTCAAAACAGCATCCAAAATGCACCTAGCAAGAGCATGTCATTCCTCTGCACAAAACTCTccaacttctctctttttttttttttttttttttttgagacggagtctcactctgtcacccaggctggagtgcaatagtgtgatcttggctcactgcaacctccacctcccagattcaagcgattctcctgcctcagcctcctgagtagctgagattacaggttcatgtcaccatgcccggctaatttttgtatttttagtagagacagggtttcaccatgttagtcaggctggtctcgaactcctgaccttgtgatccacccgcctcagcctcccaaagtgctgggattataggcatgagccaccgtgcatgacCAACTTCTCTTTTTGTTCAGAGTAAAAGCCAACGGCCCATGAGGCTTTCCATGGTCACGCCTCCGCTCATTCGCTCTGTGGCTTTGTCTTACACGGGTTCACTCCTCACTGGCCGCCTTGCTGACCCCATAGCTCACGGGCCTTACTCTGCTctcggggcctttgcacttgctccaCTGCAAATGCTCCTCCCCCAGAGGCCTTTGTGGCCCATTCCCTCGGTTCCTTAGGAACAATCCCTTCCCTGGTCAAACCTCCACTGACATCTGTCTCCTtcccttctgaattttttttctccgGTAGTATTTATCACTCTGCTATCCTTAGGATTTCCTTATCTTGTTTATCATCATCTCCTCATCCAGAGcttaagtcctttttttttttttgagatagagtctcgctctgtcgcccaggctggagtgcagtggcgcgatctcgtctcgctgaaagctccacctcccgggttcacgccattctcccgcctcagcctcccgagtagctgggactacaggcactcg", "fragmentNumber": 0, "fragmentStartPosition": 0, "fragmentLength": 2200, "numberOfFragmentsInContig": 1})
- element of array (index: 0)
- array (class [Lorg.bdgenomics.formats.avro.NucleotideContigFragment;, size 1); not retrying
Exception in thread "main" org.apache.spark.SparkException: Job aborted due to stage failure: Task 0.0 in stage 10.0 (TID 9) had a not serializable result: org.bdgenomics.formats.avro.NucleotideContigFragment
Serialization stack:
- object not serializable (class: org.bdgenomics.formats.avro.NucleotideContigFragment, value: {"contig": {"contigName": "chrUn_KN707606v1_decoy", "contigLength": 2200, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": null}, "description": "AC:KN707606.1  gi:734691250  LN:2200  rl:unplaced  M5:20c768ac79ca38077e5012ee0e5f8333  AS:hs38d1", "fragmentSequence": "ctagtagctgggactacaagcgcccgccaccacacccggctaatttttttgtatttttagtggagacaggtttcaccgtgttggccaggatggtctcgatctcctgaccttgtgatctgcccaccttgccctcccaaagtgctgggattacaggcatgagccaccatacccggcagTGTCCTATCCATTTTTAAGGCAGCCACTTGGAGTTGGAGCATGTCTTTCTCTCATAATCTCTTACCAGATGTCTCAGAGCAGCCTGTGCACTTTAACTCCAGACATTCTGCCACTGAGCCCCCTAGAGCTCCAGCTTTTAAAGCACTTGGGGTGAGCCTCGAGAGATGACAGACGGAGCTGCCCAAGAGCTGCCAGCTGCCAACCCTGCCTGGGGCTTCACGGCCCGCGCCCTACTTCCTCTCAGCTGGCTCCACACCCTGGGGCGTGTAATTTCCAAATTCTCACTCCCAGGGCTAATTTGGGGGATAAGACATTTGATTAGAAGTATCAgaaaccagctgggcatggtggctcacacctgtaatcccagcactttgggaggttatgactagaggatcatttgaactcaggaattcaagaccagcctggataacagtgagaccccatctctacaaaatataaacaattatgtgagcatggtggtgcacacctgtagtccctgttccttgggaggctgaggccggaggatcccttgagcccaggagttcaaggctgcagagagctgcgattgtgccactgcacactaacctgggagatagagcaagaacttgtctcagaaaaaaaaagtatcaggaaCTAATCTCCAGTCCTATCAAGTTAGGCATAAGGTCAATGTGTGATAGCTGAGTGTCACAGAAACCAAGGACAGGAATGCAACTGCCACTGGGGATGAACTGGAAGTGGGGAGTTAAACCACCTCAGAATGTccccatttttgtttcttctccagATGTGCTGCTTTGCTTTTCCGTATGTTTCTCTACGGACCAGCTACCTCTCCTCTGCCAACAGATCCAAGTTGTGCATGTTATGGGTCCAAACACCACGTGACAAGCCCATTCTTCCAGTTTCTCAGACCAGAAACTGCACTGTCCTCTAACTGCTTCTTCTCCCTCTTGCATCTGGTCCTTGGGGAAATCCTGTTTGCCCGGCCTTCAGCATATATCCACAGTTTAACCTTAACCACTCCTCGCCACCACTCGCGGGGGCGAGCAGCCTTCGCCCCCTGCCTAGATTACTACAGTAACTTCATTGTTCTTTCTACTTCTCTCTTTGCCCCTCTGCTATCTCAAAACAGCATCCAAAATGCACCTAGCAAGAGCATGTCATTCCTCTGCACAAAACTCTccaacttctctctttttttttttttttttttttttgagacggagtctcactctgtcacccaggctggagtgcaatagtgtgatcttggctcactgcaacctccacctcccagattcaagcgattctcctgcctcagcctcctgagtagctgagattacaggttcatgtcaccatgcccggctaatttttgtatttttagtagagacagggtttcaccatgttagtcaggctggtctcgaactcctgaccttgtgatccacccgcctcagcctcccaaagtgctgggattataggcatgagccaccgtgcatgacCAACTTCTCTTTTTGTTCAGAGTAAAAGCCAACGGCCCATGAGGCTTTCCATGGTCACGCCTCCGCTCATTCGCTCTGTGGCTTTGTCTTACACGGGTTCACTCCTCACTGGCCGCCTTGCTGACCCCATAGCTCACGGGCCTTACTCTGCTctcggggcctttgcacttgctccaCTGCAAATGCTCCTCCCCCAGAGGCCTTTGTGGCCCATTCCCTCGGTTCCTTAGGAACAATCCCTTCCCTGGTCAAACCTCCACTGACATCTGTCTCCTtcccttctgaattttttttctccgGTAGTATTTATCACTCTGCTATCCTTAGGATTTCCTTATCTTGTTTATCATCATCTCCTCATCCAGAGcttaagtcctttttttttttttgagatagagtctcgctctgtcgcccaggctggagtgcagtggcgcgatctcgtctcgctgaaagctccacctcccgggttcacgccattctcccgcctcagcctcccgagtagctgggactacaggcactcg", "fragmentNumber": 0, "fragmentStartPosition": 0, "fragmentLength": 2200, "numberOfFragmentsInContig": 1})
- element of array (index: 0)
- array (class [Lorg.bdgenomics.formats.avro.NucleotideContigFragment;, size 1)
at org.apache.spark.scheduler.DAGScheduler.org$apache$spark$scheduler$DAGScheduler$$failJobAndIndependentStages(DAGScheduler.scala:1273)
at org.apache.spark.scheduler.DAGScheduler$$anonfun$abortStage$1.apply(DAGScheduler.scala:1264)
at org.apache.spark.scheduler.DAGScheduler$$anonfun$abortStage$1.apply(DAGScheduler.scala:1263)
at scala.collection.mutable.ResizableArray$class.foreach(ResizableArray.scala:59)
at scala.collection.mutable.ArrayBuffer.foreach(ArrayBuffer.scala:47)
at org.apache.spark.scheduler.DAGScheduler.abortStage(DAGScheduler.scala:1263)
at org.apache.spark.scheduler.DAGScheduler$$anonfun$handleTaskSetFailed$1.apply(DAGScheduler.scala:730)
at org.apache.spark.scheduler.DAGScheduler$$anonfun$handleTaskSetFailed$1.apply(DAGScheduler.scala:730)
at scala.Option.foreach(Option.scala:236)
at org.apache.spark.scheduler.DAGScheduler.handleTaskSetFailed(DAGScheduler.scala:730)
at org.apache.spark.scheduler.DAGSchedulerEventProcessLoop.onReceive(DAGScheduler.scala:1457)
at org.apache.spark.scheduler.DAGSchedulerEventProcessLoop.onReceive(DAGScheduler.scala:1418)
at org.apache.spark.util.EventLoop$$anon$1.run(EventLoop.scala:48)

Process finished with exit code 1