您的位置：首页 > 其它

Quality trimming using Trimmomatic

2015-07-30 17:03 441 查看

java -jar /home/liuhui/bin/trinityrnaseq_r20140413p1/trinity-plugins/Trimmomatic/trimmomatic.jar PE -threads 20 -phred33 P_ACAGTG_L002_R1_001.fastq.gz P_ACAGTG_L002_R2_001.fastq.gz \
P_ACAGTG_L002_R1_001.fastq.gz.P.qtrim P_ACAGTG_L002_R1_001.fastq.gz.U.qtrim P_ACAGTG_L002_R2_001.fastq.gz.P.qtrim P_ACAGTG_L002_R2_001.fastq.gz.U.qtrim SLIDINGWINDOW:4:5 LEADING:5 TRAILING:5 MINLEN:25

cat P_ACAGTG_L002_R1_001.fastq.gz.P.qtrim P_ACAGTG_L002_R1_001.fastq.gz.U.qtrim > P_ACAGTG_L002_R1_001.fastq.gz.PwU.qtrim.fq
cat P_ACAGTG_L002_R2_001.fastq.gz.P.qtrim P_ACAGTG_L002_R2_001.fastq.gz.U.qtrim > P_ACAGTG_L002_R2_001.fastq.gz.PwU.qtrim.fq

gzip P_ACAGTG_L002_R1_001.fastq.gz.P.qtrim P_ACAGTG_L002_R1_001.fastq.gz.U.qtrim P_ACAGTG_L002_R2_001.fastq.gz.P.qtrim P_ACAGTG_L002_R2_001.fastq.gz.U.qtrim &

内容来自用户分享和网络整理，不保证内容的准确性，如有侵权内容，可联系管理员处理

标签：

相关文章推荐

新的分享

章节导航